Great Antibody in Dim Color for Immune Cell Flow Cytometry

December 20, 2019

Surgery
NYU Langone Medical Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC/Cyanine7 anti-mouse CD45 Antibody

Catalog Number:

103116

Clone Number:

30-F11

Excellent staining with nice separation of immune versus non-immune cells in murine pancreatic cancer.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine pancreas with orthotopically injected murine pancreas cancer cells

Primary Incubation

Incubated at 4C for 1h, dilution 1:500

Blocking Agent

1% FACS, anti-CD16/CD32

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT

Results Summary

Simple staining procedure with great staining and easy separation of CD45+ and CD45- population in murine pancreas.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Straightforward staining procedure, nice separation of populations

The Bad

Natural limitations of tandem dies

The Bottom Line

Easy-to-handle CD45 antibody with great separation despite dim color; saves brighter colors other antigens.

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