Cell Signaling Technology
Cleaved Caspase-3 (Asp175) Antibody
Polyclonal
9661
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Cell death comes in all different forms. Caspases are enzymes that mediate cell death, and cleavage of caspase-3 has been shown to be a key process. Through western blotting, we can assess this specific event.
Western Blot
Tumor cells
1:2000 in 4% BSA/TBST overnight at 4 deg
5% milk/TBST RT
1:5000 anti-Rabbit 5% milk/TBST
None
UVP imager
We are able to determine caspase-3 activity by western blotting.
N/A
Easy to use and clean antibody.
Great antibody to assess cell death via western blotting.