Abcam
Anti-LAMP2 antibody [for human]
ab25631
H4B4
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Our lab studies the role of cellular mTOR signaling in growth. Spatial organization of mTOR at the lysosome is critical for its activation. We routinely perform confocal microscopy to assess mTOR localization at the lysosome using LAMP2 as an identifying marker for lysosomes.
Immunofluorescence
HEK 293T
1:250
10% goat serum in perm buffer
1:500 anti-mouse AF555 in blocking buffer
None
Zeiss 800 Confocal Microscope
We are able to identify lysosomes in 293T cells as indicated by punctuate regions within the cell.
N/A
Perform dilution to test staining
Reliable antibody it seems as lysosome pattern is appropriate
Expensive antibody from Abcam
Good and reliable antibody to look at lysosomes in human cells.