Time Course Effect of Sirt-2 by Chemoattractant

Department of Pharmacology and Toxicology
Augusta University
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

Sirt-2

Catalog Number:

SC-28298

Clone Number:

A-5

Trauma is the leading cause of death for individuals under the age of 45 years old and it is the most common cause of preventable death in this condition. Hypotensive volume replacement permits maintenance of limited tissue reperfusion and continued metabolic activities to maintain cell viability in hemorrhagic injury. Here we checked the time course effect of Sirt-2 by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Rat tissue lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skimmed milk

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent decrease of Sirt-2 by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody is very specific and the signal is strong. Highly recommended.

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