Only Antibody to Best Stain Microglial Cells (Iba 1 from Wako)

November 25, 2019

Pharmaceutical and Biomedical Sciences
College of Pharmacy
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

FUJIFILM Wako Chemicals U.S.A. Corporation

Product Name:

Iba 1 antibody

Catalog Number:

019-19741

Clone Number:

Polyclonal

We work to evaluate the role of CNS basement membrane proteins in blood-brain barrier physiology and stroke pathology. We regularly stain microglial cells using this antibody.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain

Primary Incubation

1:200

Blocking Agent

Blocking Buffer (BSA, Donkey Serum and Triton X100)

Secondary Incubation

1:400

Tertiary Incubation

2 hr

Detection

Immunofluorescence

Results Summary

Excellent. Please use the PFA perfused mice brain sections.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great antibody

The Bad

Nothing

The Bottom Line

Sole antibody to stain microglia/macrophage cells.

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