A Good IL-13 Monoclonal Antibody PE from Thermo Fisher

December 06, 2019

Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

IL-13 Monoclonal Antibody (eBio13A), PE, eBioscience™

Catalog Number:

12-7133-82

Clone Number:

eBio13A

I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse tissue single cell suspension

Primary Incubation

1:100 dilution for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

20 minutes in 1x BD Fixation and Permeabilization buffer on ice

Tertiary Incubation

1:50 dilution on ice with intracellular staining antibodies

Detection

FACS

Results Summary

Efficient Ly-6G expression detection on mouse immune cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good antibody, strong signal and bright fluorophore

The Bad

None

The Bottom Line

Good antibody for efficient detection of IL-13 in mice immune cells.

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