Good Tollip Antibody from Thermo

October 22, 2019

cell biology
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

TOLLIP Polyclonal Antibody

Catalog Number:

PA5-30747

Clone Number:

Polyclonal

I work on various aspects of the autophagy-lysosomal system in Mammalian cells. Specially I am interrogating the substrate repertoire of autophagy using CRISPR-Cas technology as well as quantitative multiplexed proteomics. This antibody is specific and strong.

Experimental Design and Results Summary

Applications

Western Blot

Sample

human cell line, Patu-8988T

Primary Incubation

1:1000, Overnight, 4C

Blocking Agent

5% Milk, TBST

Secondary Incubation

1:10,000, anti rabbit HRP,room temp 1h

Tertiary Incubation

N/A

Detection

ECL Digital imager

Results Summary

Strong band at expected size and lost in knockout cell line indicating specificity

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific

The Bad

Looks like it might loose potency after multiple freeze thaws. would suggest using fresh

The Bottom Line

Would recommend for western applications.

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