eBioscience (Thermo Fisher Scientific - Invitrogen)
CD8a Monoclonal Antibody (OKT8 (OKT-8)), Alexa Fluor 488, eBioscience™
53-0086-42
OKT8 (OKT-8)
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Browse Similar Products: CD8a Flow Cytometry Antibodies
Our research involves the study of immune cell phenotypes in different disease states with a focus on viral infection. This antibody was used to assess the purity of CD8+ T cells that were positively selected from total peripheral blood mononuclear cells.
Flow Cytometry
Purified CD8+ T cells from human PBMC by magnetic bead-based positive selection
2.0x10^5 cells were stained with the manufacturer's recommended volume of antibody in a total staining volume of 100ul autoMACS running buffer (Miltenyi Biotec) for 30 minutes on ice in the dark.
10 min on ice with human Fc receptor blocking reagent (Miltenyi Biotec) prior to primary incubation with fluorochrome-labelled antibody
N/A
Becton Dickinson FACSCalibur flow cytometer
This antibody gives a distinct peak of CD8+ cells (unfilled histogram) that are easily distinguished from CD8+ cells stained with a matched isotype control antibody (filled histogram).
10.1186/s12865-015-0113-0
Bright staining and little optimization required.
None
This is an excellent antibody for identifying CD8+ cell populations or analyzing CD8 expression levels in peripheral blood mononuclear samples using flow cytometry.