Abcam
Anti-RPL28 antibody
ab138125
Polyclonal
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I am a chemical biologist, and my research focuses on identifying small molecules that selectively target cellular processes involved in maintaining protein homeostasis. Therefore, I perform chemical, biochemical, and cell biological assays relevant to protein synthesis (ribosome) and degradation (the ubiquitin proteasome system and autophagy). This antibody is good to probe RPL28, but it has some background bands.
Western Blot
HCT116 human cell line
1:2000 dilution in 5% milk
5% milk
1:5000 rabbit HRP
N/A
Biorad Chemidoc, ECL
I got the thick band around 18kDa region. However, there were 2-3 off-target bands above 18kDa as well.
Intensity of the band
Some off-target bands
Okay antibody to detect RPL28.