Cell Signaling Technology
FIP200 (D10D11) Rabbit mAb
12436
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I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. For this FIP200 antibody, KO clones were validated; however, it was not easy to detect FIP200, as the band was too weak.
Western Blot
HCT116 cell lysate
1:1000 in 5% BSA in TBST, overnight incubation at 4 degree
5% non-fat milk in TBST
1:20000 Licor IRdye800 rabbit
Washed with TBST three times, 5 min each
Odyssey clx
I used this antibody to screen FIP200 KO. Since the signal was quite weak, I had to load great amount of the lysate in order to validate clear knock-out.
N/A
Okay selectivity
Low sensitivity
I would try another antibody first before trying this one.