Santa Cruz Biotechnology Inc
Tubulin (5F131)
sc-73242
5F131
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Browse Similar Products: Tubulin Western Blot Antibodies
Human cells were growth-arrested, treated with a specific growth factor; protein extracts were made and resolved in a 10% SDS PAGE. Resolved proteins were western transferred in the nitrocellulose membrane and immunodetected for STAT1 protein levels. For normalisation of the blot, the same membrane was reprobed with anti- Tubulin antibody which was obtained from Santacruz Biotechnology Inc. Selection of antibody was based on the information provided in the flyer.
Western Blot
Human cell protein extract
Dilution (1:500) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk made in 1x TBST
Goat anti-mouse IgG HRP, dilution (1:4000) in 5% skimmed milk made in 1x TBST, 1hr at RT
None
Enhanced chemiluminescent detection in X-Ray films
Indicated anti-tubulin antibody from Santacruz Biotechnology Inc has detected only one specific band of tubulin at the correct size. However, the signal was too low, and the membrane was full of patches may be due to some impurities, which leads to nonspecific binding of primary Ab to the nitrocellulose membrane. This conclusion was made by performing the same experiment at least 4 times with the same antibody.
N/A
Gives single band
A lot of non-specificity in the form of patches.
Poor antibody