Thermo Fisher Scientific
Cyclin E2 Polyclonal Antibody
PA1-28367
Polyclonal
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To test the time course effect of particular growth factor on cyclin E2 expression in mouse, the primary cell lines were growth-arrested for 12 hrs in medium devoid of FBS. The synchronised cells treated without or with growth factor for an indicated periods were employed to prepare proteins extracts, and an equal amount of proteins were resolved in 10%SDS-PAGE. The resolved proteins were then western transferred in a nitrocellulose membrane and probed with cyclin E2 specific antibody. Although, Thermo Fisher Scientific supply a polyclonal antibody, the selection of Ab was based on the merits indicated in antibody flyer.
Western Blot
Mouse primary cell whole cell lysate
Dilution (1:500) in 5% BSA prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk made in 1x TBST
Goat anti-rabbit IgG HRP, dilution (1:4000) in 5% skimmed milk prepared in 1x TBST, 2hr at RT
None
ECL detection in X-Ray films
Although Cyclin E2 from Thermo Fisher Scientific was a polyclonal antibody, it has detected only a specific band at the correct molecular weight (compared with low range protein marker, Bio-Rad# 161-0304). Interestingly, a highly specific band with high Intensity was observed upon growth factor treatment.
N/A
Highly specific for cyclin E2.
Antibody never cross-reacted with other nonspecific epitopes
Excellent antibody for the detection