A Very Good Myosin Heavy Chain II-B Antibody from BioLegend

Johns Hopkins University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Purified Non-muscle Myosin Heavy Chain II-B Antibody

Catalog Number:

909901

Clone Number:

Poly19099

I seek to understand the molecular mechanisms underlying development of the mammalian nervous system. Specifically, this includes understanding how neurons generate their complex morphology and form proper circuitries during development, and how neurons regenerate to restore connections after brain or spinal cord injuries. In this experiment, I wanted to detect the expression of non-muscle myosin heavy chain II-B in mouse CAD cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse CAD cells

Primary Incubation

1:1000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:2000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

This antibody works well in western blot experiment.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody is specific and sensitive

The Bad

N/A

The Bottom Line

A very efficient antibody.

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