TNFa Production and Release in Response to LPS

July 22, 2019

Medical University of Graz
Stiftingtalstraße 24
Postdoctoral Researcher

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Company:

Novus Biologicals

Product Name:

TNF-alpha ELISA Kit

Catalog Number:

NBP2-31085

TNFa is one of the major inflammatory cytokines. Treatment of HL-1 cardiomyocytes with LPS (1 microg/ml) resulted in an increase in extracellular TNFa levels, as measured by this kit. Empagliflozin showed protective anti-inflammatory potential against LPS-induced TNFa release.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

Cardiomyocytes

Tips

N/A

Results Summary

LPS induces TNFa production and release in cardiomyocytes, whereby empagliflozin showed beneficial effects.

DOI or PMID #

N/A

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Summary

The Good

Worked as expected

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The Bottom Line

This kit can be used to estimate TNFa levels in cell culture medium and plasma of mouse origin.

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