Thermo Fisher Scientific
Anti-CD11c Mouse Monoclonal Antibody, Biotin
13-0114-82
N418
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To detect dendritic cells in mouse splenocyte and PBMCs, we have used CD11c biotin antibody.
Flow Cytometry
Splenocytes, peripheral blood mononuclear cells
1:100 at 4 degree C for 30 mins
Fc-block
Incubated with streptavidin PE at 1: 200 dilution
None
Flow cytometry
Clear cloud for CD11c positive cells were observed.
N/A
Use any fluorochrome streptavidin antibody, which works in your cocktail
Easy to optimize, can be used at higher dilutions
Good biotinylated antibody to detect CD11c surface marker present on dendritic cells.