R&D Systems
Recombinant Mouse M-CSF Protein
416-ML
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Microglia were isolated from adult mice and kept in culture. Medium was supplemented with 10ng/ml M-CSF and it refreshed after 3 days.
Cell Culture
Adult murine microglia cells
Nothing specific
Medium was supplemented with 10ng/ml M-CSF was crucial in order to help microglia ramification and general viability in culture.
N/A
Easy to use and works as expected
None
A good and basic growth factor for microglia cultures.