Thermo Fisher Scientific
V5 Tag Monoclonal Antibody
R960-25
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I specialize in functional genomics and applying gene editing technologies to address immunology questions. I have recently developed a new barcoding system, which represents the largest known to date set of protein-based reporters (doi.org/10.1016/j.cell.2018.09.022). I used this product for detection of V5-tagged recombinant protein expressed by HEK-293T cells.
Mass cytometry
Human HEK-293T cells transduced with V5-tagged recombinant protein
1:1000 (200 ug/ml stock), 30 min on ice
Human Fc blocking reagent (BioLegend)
N/A
CyTOF 2
Excellent detection of V5-tagged protein; low background signal. For mass cytometry, antibody was directly conjugated with a metal isotope prior to staining. Works well also for flow cytometry and WB.
doi.org/10.1016/j.cell.2018.09.022
High specificity, very sensitive
Excellent antibody, highly recommended!