Tocris Bioscience
PF 8380
4078
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During preliminary studies we investigated whether the ATX inhibitor PF-8380 crosses the BBB and impacts LPA levels in the brain. After oral gavage of 3mg/kg PF-8380 per mouse (PF-8380 was dissolved in hydroxyl methyl cellulose), mice were anesthetized and perfused with PBS at different time points and the whole brains were dissected and immediately snap frozen in liquid nitrogen. Lipids were extracted using the Bligh & Dyer HCl method and the levels of LPA and PF-8380 were quantitated by LC-MS/MS.
In vivo, LC-MS/MS
Mouse brain
If hard to dissolve, leave it stirring overnight or add some µl of DMSO (in case that it doesn't affect your experimental procedure)
PF 8380 does cross the BBB and decreases the levels of lysophosphatidic acid in mouse brain.
N/A
Easy to use and works as expected
Hard to dissolve sometimes.
A very good and potent ATX inhibitor.