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APC/Fire™ 750 anti-human CD11b Antibody
301352
ICRF44
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This antibody has been used to optimise flow cytometry-based immunophenotyping of whole-blood samples. The results have not yet been published. Multi-colour antibody panels were designed to identify a number of lymphocyte populations and tested to ensure appropriate compensation was possible. CD11b was used in a ten-colour panel to help distinguish myeloid derived suppressor cells (MDSCs). It is easy to use and gives clear staining.
Flow Cytometry
Human whole blood
3µL APC/Fire750-CD11b for 30 minutes at 4°C
FcR blocking reagent, human (Miltenyi Biotec, cat# 130-059-901)
N/A
Beckman Coulter Gallios, Kaluza Software
Clear staining that, in combination with HLA-DR, helped identify myeloid derived suppressor cells (MDSCs). Titration optimisations showed we could reduce the volume of antibody used with no detriment to the results. Prior to analysing samples, erythrocytes were lysed using 1.5 mL 1xPharmLyse (BD, cat#555899) at room temperature for 30 minutes. This antibody fit well into a ten-colour panel with no compensation issues.
Clear staining of CD11b+ cells that is useful for FACS analysis.
The antibody has only been tested for flow cytometry.
An easy-to-use antibody that provided good clear staining.