BioLegend
PE anti-human CD62L Antibody
304805
DREG-56
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This antibody has been used to optimise flow cytometry-based immunophenotyping of whole blood samples. The results have not yet been published. Multi-colour antibody panels were designed to identify a number of lymphocyte populations and tested to ensure appropriate compensation was possible. This antibody was used within a ten-colour staining panel to distinguish subpopulations of T cells. The antibody is easy to use and gives clear staining.
Flow Cytometry
Human whole blood
2µL PE-CD62L for 30 minutes at 4°C
FcR blocking reagent, human (Miltenyi Biotec, cat# 130-059-901)
N/A
Beckman Coulter Gallios, Kaluza Software
Staining allowed the distinction of memory, effector and naive populations of CD4+ T. The antibody was titrated and has been used at a lower volume than that suggested by the manufacturer. Prior to analysing samples, erythrocytes were lysed using 1.5 mL 1xPharmLyse (BD, cat#555899) at room temperature for 30 minutes. This antibody fit well into a ten-colour panel with no compensation issues.
Good for identifying populations of memory, effector and naive T cells.
The antibody has only been tested for flow cytometry.
Easy-to-use antibody pack that is easily used in multi-colour assays.