Santa Cruz Biotechnology
arginase I Antibody (E-2)
sc-271430
E-2
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Primary murine microglia cells were incubated in the absence or presence of LPA (1 μM) for 24 and 48 h. Cells were stained for specific inflammatory markers (in this case with Arg1) and nuclei were counter stained with Hoechst. The fluorescence intensity was quantitated using ImageJ.
Immunofluorescence
Primary murine microglia
1/100, O/N at 4°C
5% BSA
Cy3 anti-mouse, 1/300, 30min at 4°C
N/A
Confocal Microscopy
In time-dependent studies, Arg-1 expression was reduced in response to LPA treatment.
10.1186/s12974-016-0701-9
Easy to use, reproducible results, very good price
Doesn't work well in WB.
Good antibody for the detection of Arg1 (in ICC worked perfectly, however it didn't work well in WB).