Visualization of Microtubules Network in Microglia

Molecular Biology and Biochemistry
Medical University of Graz
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (FITC)

Catalog Number:

ab64503

Clone Number:

DM1A

BV2 or primary murine microglia were stained for Iba-1 (red) and alpha Tubulin (green) in order to observe possible changes in the microtubules network.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

BV2 and primary murine microglia

Primary Incubation

aTub: 1/1000, Iba-1: 1/300, 24 hours at 4°C

Blocking Agent

5% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Confocal Microscopy

Results Summary

In BV2 we observed a more localized staining around the nucleus. In primary microglia the staining expanded in the cytoplasm.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very clear and reproducible results

The Bad

None

The Bottom Line

A very good Ab for the visualization of alpha Tubulin.

Share your experience with other scientists. Write a Review! »

Join the discussion