MilliporeSigma
Monoclonal Anti-vimentin
V2258
LN-6
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Browse Similar Products: Vimentin Immunofluorescence Antibodies
I have used anti-vimentin antibody on breast cancer cells to determine the epithelial to mesenchymal transition in these cells. The source of identification is immunofluorescence staining followed by detection by imaging on the confocal microscope. Anti-vimentin from sigma is good positive control for mesenchymal cells, especially triple-negative breast cancers with a mesenchymal phenotype .
Immunofluorescence
Human breast cancer cells
1:200 at 4 degree for overnight
Donkey serum
1:100 at room temperature for 1 hour
N/A
Confocal microscope
Very clearly shows the difference between the mesenchymal and epithelial breast cancer cells by the presence or absence of vimentin. I have used this antibody in a 3D-coculture system, this antibody binds specifically to human but not the mouse cells present in the coculture system.
You can use it low concentrations
You can use this antibody for IF staining on mesenchymal TNBC cells, also use as a positive control.