Santa Cruz TGFß1 Antibody Works Well

Pediatrics
Drexel University
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Crutz Biotechnology

Product Name:

TGFβ1 Antibody (3C11)

Catalog Number:

sc-130348

Clone Number:

3C11

TGF-β1 involves in proliferation, angiogenesis, and promotion or inhibition of many immune events. TGF-β1 is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells. One of my experiments needs to measure the expression of TGF-β1. I used this antibody for Western blot and it worked very well for me.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lung tissue

Primary Incubation

TGF β1 (1:500 dilution), Overnight at 4 degree on rocking shaker

Blocking Agent

Odyssey blocking buffer (1:1)ratio with1X PBS buffer

Secondary Incubation

Goat anti-Mouse IgG, LICOR (1:5000 dilution) at RT for 1hr

Tertiary Incubation

N/A

Detection

Scanned on Odyssey® CLx (auto scan 700 & s800).

Results Summary

Anti TGF β1 is a good antibody. I recommend this antibody for WB.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

My results were satisfactory

The Bad

N/A

The Bottom Line

I would highly recommend this antibody.

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