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IgG (minimal x-reactivity) Antibody
406416
Poly4064
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I am working with a multiplex immunofluorescence technique in which you can stain one single tissue section with more than 100 antibodies. I have a portfolio of more than 2000 different antibodies and have generated over 100,000 immunofluorescence images. I supervise several projects in which we stain different tissue samples, e.g., skin (melanoma, psoriasis), tumor, spleen, etc. Our focus for this project was to characterize the germinal center in a B cell-specific CD83 conditional knockout model as CD83 modulates B cell activation and germinal center response.
Immunofluorescence
Murine spleen
1:50; RT; 60 min
5% FBS; RT, 30 min
None
Fluorescence signals (AlexaF 488) were detected by an inverted fluorescence microscope (Leica DM IRE2)
This antibody gives very specific and strong signals on frozen-tissue sections with no background. Publishable results!
N/A
Specific signals, no background!
Also a great antibody for IF (not only FC)