Good Brilliant Violet 605™ Anti-Human CD117 Antibody from BioLegend

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 605™ anti-human CD117 (c-kit) Antibody

Catalog Number:

313218

Clone Number:

104D2

CD117, also known as c-Kit, is a receptor for stem cell factor (SCF) or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells, and innate lymphoid cells, mainly, but not solely, restricted to ILC3s.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

15 min, 37°c

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

Great, bright, and sensitive antibody for CD117 expression in both adaptive lymphocytes and innate lymphoid cells in various compartments. Can also be used to identify hematopoietic progenitor cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright, sensitive, easy to use

The Bad

Must be used with other markers to increase resolution of positive vs negative populations.

The Bottom Line

Would recommend

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