Awesome Kip2 Antibody from Boster Bio

May 21, 2019

Pathology
Johns Hopkins University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Boster Bio

Product Name:

Anti-p57 Kip2 Rabbit Monoclonal Antibody

Catalog Number:

M01244-1

Clone Number:

BGD-3

We've been using this Ab for several months, and it's been working great. We have already ordered several vials of this Ab for detecting podocytes in mice kidney.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mice kidney

Primary Incubation

1:100, room temp, 90 minutes

Blocking Agent

Peroxidase blocker

Secondary Incubation

Mouse/Rabbit Unovue, 30 minutes, rom temperature

Tertiary Incubation

N/A

Detection

DAB chromogen

Results Summary

We used an autostainer machine for the process. With this Ab dilution, and using EDTA for Ag retrieval, we had the least background.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use, works every time

The Bad

In rare occasions it stained parietal cells in the glomeruli

The Bottom Line

Worth it.

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