This BioLegend Antibody Stains CD90.2 Cells Very Well

May 14, 2019

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 785™ anti-mouse CD90.2 Antibody

Catalog Number:

105331

Clone Number:

30-H12

We wanted to study how T cells responded to stimulation in vitro, so we used this antibody to identify T cells in the culture.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stained CD90.2+ T cells very clearly in the culture.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody stains T cells very clearly

The Bad

None to mention

The Bottom Line

The antibody is great for distinguishing T cells in a single-cell suspension.

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