Ki 16425 Inhibits LPA-Mediated COX-2 Activation

Medical University of Graz
Division of Cardiology
Postdoctoral Researcher

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Company:

Tocris Bioscience

Product Name:

Ki 16425

Catalog Number:

5056/5

MG-63 cells were pre-incubated with 10 µM Ki 16425 for 30 min followed by treatment with 10 µM LPA for 3 h to follow COX-2 expression using Western blot. Ki 16425 inhibited COX-2 induction by LPA.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

MG-63 cells

Tips

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Results Summary

MG-63 cells were pre-incubated with 10 µM Ki 16425 for 30 min followed by treatment with 10 µM LPA for 3 h to follow COX-2 expression using Western blot. Ki 16425 inhibited COX-2 induction by LPA.

DOI or PMID #

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Summary

The Good

Worked as expected

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The Bottom Line

Ki 16425 inhibited COX-2 induction by LPA in MG-63 osteosarcoma cells

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