Cell Signaling Technology
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP Rabbit
4858
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Our lab studies the role of mTOR signaling in T cell such as mTORC1 signaling during T cell differentiation and function. With immunoblot analysis, we routinely assess pS6 (S235.236) activity as a direct readout of mTORC1 signaling in WT T cells upon T cell activation.
Western Blot
Murine CD8+ T cells
1:10000 in 4%BSA/TBST 4deg overnight with shaking
5%milk/TBST for 1hr room temperature with shaking
1:5000 anti-Rabbit HRP (CST) in 5%milk/TBST for 1hr room temperature with shaking
None
UVP Imaging System
We are able to detect mTORC1 activity by assessing pS6 (S235.236) in WT T cells upon stimulation through immunoblot analysis.
N/A
Titrate antibody since it can be quite bright depending on cell type and activity
Great monoclonal antibody to detect mTORC1 signaling. Can reuse antibody at least 10 times plus.
Easy-to-use antibody to analyze mTORC1 activity via western blotting.