Phospho-p44/42 MAPK Antibody for Western Blot from CST

R & D
Aptose Biosciences
Senior Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101

Catalog Number:

9101

In our lab, we aim to study a small molecule kinase inhibitor’s activities in various cell lines by investigating phosphor-proteins of interest expression levels. This antibody that we used is very reliable for Western blot application. No optimization required.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates

Primary Incubation

1:1000, overnight, four degrees Celsius

Blocking Agent

Licor blocking solution

Secondary Incubation

1:5000, 2 hours, room temp

Tertiary Incubation

None

Detection

Fluorescence imaging

Results Summary

This antibody yielded bands at the correct size, without unspecific binding and background issues.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use, reliable results

The Bad

None

The Bottom Line

This is a great antibody for western blotting. Gave results as expected.

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