Cell Signaling Technology
Phospho-FLT3 (Tyr591) Antibody #3461
3461
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In our lab, we aim to study a small molecule kinase inhibitor’s activities in various cell lines by investigating phosphor-proteins of interest expression levels. This is antibody that we used is reliable and easy to use. Following CST standard protocol yielded consistent results.
Western Blot
Cell lysates
1:1000, overnight, four degrees Celsius
Licor blocking solution
1:5000, 2 hours, room temp
None
Fluorescence imaging
Very consistent results each time. See clear bands at the correct places, not much background.
N/A
Easy to use, reliable results
This is a good antibody for western blot application.