Abnova
Ppp1r3c purified MaxPab rabbit polyclonal antibody (D03P)
H00005507-D03P
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: PPP1R3C Western Blot Antibodies
We tried using this antibody to detect Ppp1r3c (PTG) protein from lysates of mouse primary hippocampal cultures, mouse primary spinal cord cultures, lysates of dorsal spinal cord, and lysates of nominally pure primary astrocyte cultures, with antibody dilutions down to 1:250 and O/N RT incubation. Under no conditions could we detect the native protein.
Western Blot
Mouse, primary hippocampal cultures, primary spinal cord cultures, nominally pure primary astrocyte cultures, dorsal spinal cord tissue
1:250 - 1:1000, O/N incubation at RT in 5 % milk in PBS-T
5 % milk
1:5000 HRP-conjugated Gt anti-Rb, 30 min at RT in 5 % milk in PBS-T
None
ECL (BioRad Clarity Max), Chemiluminscence Imager
No bands of native protein detected. Positive controls: GFAP detected, overexpressed human Ppp1r3c detected.
N/A
Nothing
It didn't work
Only useful for detecting overexpressed Ppp1r3c. Not useful for detecting native mouse Ppp1r3c.