DISC1 Antibody for Dendritic Spine Analysis

April 11, 2019

Physiology
Jali Medical Inc.
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

DISC1 Polyclonal Antibody

Catalog Number:

40-6800

Disrupted in Schizophrenia 1 (DISC1) is a protein involved in cytoskeletal regulation. For this review, the antibody was used to establish a relationship between changing dendritic spine structure and disease models and associated behaviors. The antibody produces high resolution images with obvious staining clusters.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse hippocampal brain tissue

Primary Incubation

N/A

Blocking Agent

Blocked in 0.01 M PBS supplemented with 1% bovine serum albumin, 0.25% Triton and 0.05% sodium azide for 2 hr

Secondary Incubation

Primary Ab in .01 M PBS supplemented with 1% bovine serum albumin, 0.25% Triton and 0.05% sodium azide for 2 hr

Tertiary Incubation

Secondary Ab in in 0.01 M PBS overnight

Detection

Fluorescence

Results Summary

A simple protocol was used producing good resolution images. This is one of the most used DISC1 antibodies in the market and the optimal dilution based on much cited work is 1:400/1:500. The antibody does not require much further optimization. The fluorescent intensity of the images is exceptional even if only a few clusters of proteins are detected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Consistent, good staining

The Bad

Minor instances of background staining

The Bottom Line

Excellent quality stain enabling visualization for colocalization in the dendritic spine, max size of 10 microns. Clusters of DISC1 protein are obvious with magnification of 60x2.

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