Great SOX10 Antibody for Oligodendrocyte Lineage

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

SOX10 Antibody (CL4455)

Catalog Number:

NBP2-59050

The aim of our research is to study a role played by oligodendrocytes in neurodegenerative diseases. The specific goal of this experiment was to quantify oligodendrocytes in the course of disease progression. This antibody demonstrated excellent staining for oligodendrocytes.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse optic nerve

Primary Incubation

Diluted at 1:100, overnight at 4°C

Blocking Agent

10% normal donkey serum + 0.5% triton X + 0.1M PBS

Secondary Incubation

Alexa 488 conjugated anti-mouse IgG 1:500, 1 hr at RT

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

This antibody clearly stained oligodendrocytes in mouse optic nerve tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Robust staining with no background or unspecific binding

The Bad

Relatively expensive

The Bottom Line

Highly recommended as a oligodendrocyte marker in central nerve system

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