Tecan Microplate Reader for Quantifying Bacterial Fluorescence

The Tecan Infinite 200 Pro microplate reader is an effective instrument for quantifying a wide range of bacterially expressed fluorescent proteins over diverse growth conditions. As an example, I've utilized this instrument to quantify the levels of a specific small molecule in bacterial supernatants using a dual-color fluorescent bacterial biosensor strain. This ability to image both absorbance and fluorescence at different wavelengths, while incubating a sample under optimal conditions of temperature and agitation, allowed for maximal dynamic range of my readout.