The Good
It produces a strong signal with a broad dynamic range. There is little background fluorescence in living D. discoidium cells. The assay is very rapid, and cell death can be imaged immediately.
The Bad
It can degrade or bleach if not kept in the proper conditions. There can be background spots that would affect fluorescent quantification. Also quantification of mean fluorescence per cell can be variable. This product is idea for a binary live/dead assay.
The Bottom Line
I highly recommend this product for assaying cell death of this organism. It is versatile and easy to use.