Tocris Bioscience
FURA-2AM
2220/1
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FURA-1AM is a calcium sensitive dye that gives fluorescence in calcium-bound and -free states at 2 different wavelengths.
Cell culture
Primary guinea pig ventricular cardiomyocytes
Primary guinea pig ventricular cardiomyocytes were loaded with FURA-2AM (1 μM) and Pluronic F 127 (1%) for 30 min followed by washing with normal-Tyrode solution.
N/A
Cells were stimulated at 1 Hz frequency and fluorescence intensities were measured.
Worked as expected
FURA-2AM can be used for radiometric intracellular calcium homeostasis measurements.