CD8a Antibody from BioLegend

Biology and Biochemistry
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-mouse CD8a antibody

Catalog Number:

100722

Browse Similar Products: CD8a Flow Cytometry Antibodies

This antibody was used in flow cytometry analysis to detect T-cytotoxic cells in spleen and tumor samples.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes and solid tumors

Primary Incubation

Antibody was diluted at 1:200 in FACS staining buffer for 20 minutes on ice

Blocking Agent

Truestain FcX at 1:50 dilution in FACS staining buffer

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS

Results Summary

Our research program involves profiling of lymphocytes in spleen and tumors. To detect T-cytotoxic cells from spleen and tumor samples, we stained these samples with anti-CD8 antibody. This antibody worked really well in separating T-cytotoxic cells from T-helper and T-Reg cells with no difficulty in optimization.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very good antibody

The Bad

Nothing

The Bottom Line

Good antibody to detect T-cytotoxic cells. I highly recommend this antibody for Flow cytometry studies.

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