PE Anti-Mouse CD120a (TNFaR Type I/p55) Antibody

January 31, 2019

Advanced Diagnostics
University Health Network
Postdoctoral Fellow

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Company:

BioLegend

Product Name:

PE anti-mouse CD120a (TNFaR Type I/p55) Antibody

Catalog Number:

113003

We used this antibody for identification of cytokine receptor expression in splenic neutrophils. The antibody specifically labels CD120a but is not very bright.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

15 minutes

Blocking Agent

Anti-CD16/CD32 antibody

Secondary Incubation

30 minutes

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

TNF1Ra expression on the surface of splenic neutrophils can be identified using this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It specifically labels CD120a.

The Bad

It does not provide a very bright signal.

The Bottom Line

PE CD120a is a good antibody to label TNFRa expression in splenic neutrophils.

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