Very Good Mouse TCR Vß5.1, 5.2 Antibody

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse TCR Vβ5.1, 5.2 Antibody

Catalog Number:

139504

Our lab studies the function of T cells in an influenza model in mice, and to do so we use various genetically modified mice. In one of our models, we need to be able to identify the Vβ5 T-cell receptor. We chose this antibody because it’s very bright so we can detect these molecules even at low levels.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood

Primary Incubation

Cells were stained in 100 µL FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100 µL FACS buffer.

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

This antibody is very bright with great separation so we can clearly identify the Vβ5 TCR cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and stable

The Bad

NONE

The Bottom Line

This antibody works efficiently to identify the mouse Vβ5 TCR.

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