The Antibody Identifies CD5+ Cells Very Well

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 488 anti-mouse CD5 Antibody

Catalog Number:

100612

We wanted to study B cells in the diseased vs healthy, so we used this antibody to separate the CD5- and CD5+ B cell populations in the peritoneum. The antibody works well and is clear.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of peritoneal cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody clearly differentiates the CD5- and CD5+ B cell populations in the perigoneum.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains CD5+ cell very clearly.

The Bad

None to mention

The Bottom Line

The antibody is great for differentiating the CD5- and CD5+ B cell populations.

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