The Antibody Identifies CD27+ Cells Very Well

January 04, 2019

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 488 anti-mouse/rat/human CD27 Antibody

Catalog Number:

124222

We wanted to study CD27+ cells in the disease vs healthy state, so we used this antibody to identify CD27+ cells. The antibody works well to identify CD27 in B and T cells in the spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody identifies CD27+ cells in the mouse spleen very well.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains CD27+ cells very well.

The Bad

None to mention

The Bottom Line

The antibody identifies CD27+ B and T cells in the spleen well.

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