Cell Signaling Technology
Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
2605
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In the present study, pPAK1 + pPAK2 levels were measured in the muscle of rats subjected to ischemia. For this, male albino Wistar rats' femoral arteries were ligated and cut, and after 7 days of recovery, femoral muscles were harvested. Proteins were extracted from samples resolved in SDS-PAGE and immunoblotted for phospho-PAK1/PAK2 levels. The antibody was selected based on previous experience of the same antibody with human samples, and it works well in rat, too.
Western Blot
Whole tissue protein lysate
Overnight at 4°C (5% BSA in TBS with 0.1% Tween 20; dilution 1:1500)
5 % Skimmed milk in TBS with 0.1% Tween 20 (for 2 hr at RT)
2 hr at RT (5% skimmed milk in TBS with 0.1% Tween 20; dilution 1:4000)
None
ECL
The cell signalling phospho-PAK1/PAK2 antibody has given a very specific band of pPak 2 and Pak1/3 at the correct size. However, there was a significant change in pPak2 levels in response to treatments.
N/A
Antibody is highly specific to phospho-PAK1/PAK2
A nonspecific band was also observed at 26 kDa
Excellent specificity of phospho-PAK1/PAK2 antibody in rat samples