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Alexa Fluor 700 anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody
202528
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To analyze cell-intrinsic properties of certain proteins, we routinely perform adoptive transfer experiments of congenically different transgenic T cells (CD90.1) into WT B6 (CD90.2) mice upon infection. By staining for CD90.1 and CD8 expression, we are able to distinguish transferred cells from host CD8 T cells. This a great antibody that is really bright.
Flow Cytometry
Murine transgenic T-cells during an infection
1:2000 in FACS buffer for surface staining
FC block 100 µg/mL in FACS buffer
None
BD Celesta flow analyzer
We are able to detect the adoptively transferred cells from the host CD8 T cells. The population is quite distinct.
N/A
Make sure to titrate antibody depending on analyzer.
Really bright antibody and good dilution saves antibody usage over time.
Can be tricky and too bright if not titrated for cell conditions.
Easily the best antibody for congenic staining.