Thermo Fisher Scientific
IFN gamma Monoclonal Antibody (XMG1.2), PerCP-Cyanine5.5, eBioscience
45-7311-82
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As an immunologist, we assess CD8 T cell cytokine secretion via flow cytometry. We analyze interferon gamma secretion (IFNg) since this cytokine is one of the first cytokines to be expressed for cytolytic activity. Upon stimulation, we can detect expression of this cytokine in CD8 T cells. The antibody is great to detect IFNg expression in T cells.
Flow Cytometry
Murine T-cells during an infection
1:500 in 1x BD perm/wash buffer after surface staining and fix/perming
FC block 100 µg/mL in FACS buffer
None
BD Celesta flow analyzer
We are able to detect IFNg expression in T cells by re-stimulating T cells in the presence of a golgi inhibitor to prevent secretion.
N/A
Make sure to surface stain then fix/perm (BD Bioscience) then stain ICS in 1x Perm Wash
Really good and bright antibody to detect IFNg expression along with other cytokines.
None whatsoever
Great antibody and clone to detect IFNg expression in T cells upon stimulation.