Great Antibody to Detect Granzyme B Expression in T Cells

January 15, 2019

Oncology
Johns Hopkins School of Medicine
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor 647 anti-human/mouse Granzyme B Antibody

Catalog Number:

515406

We routinely assess cytolytic activity in CD8 T cells upon acute infections. Granzyme B is a canonical marker for this activity using flow cytometry. Along with other cytokines, granzyme B indicates a cell with polyfunctional capability and superior ability to kill target cells. The antibody is good and bright.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine T-cells during an infection

Primary Incubation

1:250 in 1x BD perm/wash buffer after surface staining and fix/perming

Blocking Agent

FC block 100 µg/mL in FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD Celesta flow analyzer

Results Summary

We are able to easily detect Granzyme B secretion in CD8 T cells from murine T cells during an infection.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very bright signal and can easily detect the population

The Bad

Higher dilution than other cytokine antibodies we use

The Bottom Line

One of the best antibodies to detect Granzyme B in T cells. We went through multiple trials before we came across this clone and color.

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