Magnetic Sorting with Anti-Mouse CD117 Microbeads

Weill Cornell Medical College
Pharmacology
Lab Specialist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Miltenyi Biotec

Product Name:

CD117 MicroBeads, mouse

Catalog Number:

130-091-224

It has been shown that CD117+ is a marker of prostate basal stem cells. We used this anti-mouse CD117 magnetic microbeads to sort cells from the prostate, cells that constitute the putative population of basal cells that can reconstitute prostatic ducts. This research aimed to characterize the cell type(s) in the prostate that express a key marker of murine and human ESCs, and explore the hypothesis that aberrant expression of this marker may contribute to prostate inflammation/hyperplasia sometimes associated with prostatitis. We recommend this product.

Experimental Design and Results Summary

Application

Magnetic sorting with anti-mouse CD117 microbeads

Starting Material

Isolated murine prostate cells

Protocol Overview

Protocol as described in (Leong K.G., Wang B.E., Johnson L., and Gao W.Q. Generation of a prostate from a single adult stemcell. Nature. 2008;456(7223):804-8.)

Tips

Extending incubation time can be helpful

Results Summary

Sorted CD117+ cell populations as described in Abstract

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Technical support from Miltenyi is very good

The Bad

Price point is on higher end

The Bottom Line

We would recommend. Quality marker for use in MACS/Flow.

Join the discussion