Anti-HA Tag Antibody to Detect Overexpression of Protein of Interest

December 19, 2018

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

MilliporeSigma

Product Name:

Anti-HA antibody produced in rabbit

Catalog Number:

H6908

Browse Similar Products: HA Tag Western Blot Antibodies

We used the HA-tag antibody to detect different mutant proteins. There were some non-specific bands but the antibody is still a great antibody.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MCF-7 overexpression of HA-tag proteins

Primary Incubation

Primary antibody against HA

Blocking Agent

5% milk

Secondary Incubation

Anti-rabbit HRP

Tertiary Incubation

None

Detection

ECL substrate

Results Summary

Able to detect different mutant expressions of HA-tag protein.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very good detection

The Bad

Some non-specific bands in cells that don't express the tag.

The Bottom Line

Still a really great antibody to detect HA-tagged protein.

Share your experience with other scientists. Write a Review! »

Join the discussion