eBioscience/Invitrogen
eBioscience™ Cell Proliferation Dye eFluor™ 450
65-0842-90
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We are interested in studying the mechanism of T cell differentiation through modulating the mTOR pathway. This cell proliferation dye is a good dye that is able to distinguish at least 5 cell divisions.
Flow cytometry
Mouse whole splenocytes
Cells were labeled in PBS in 10e6 cells/mL in 10 µM at 37°C for 10 min. Then cells where washed and activated for 72 hrs to look at proliferation.
Nothing particular
Able to detect at least 5 divisions.
N/A
Minimal spillover to any channels on flow cytometry
Make sure you do not overstain cells. Viability can be affected.
One of the best proliferation dyes with minimal toxicity.